Arraystar inc rat lncrna microarray
$(A) Experimental setup to detect the deregulated <t>lncRNA</t> in hearts subjected to HF, HF+AAV9-SERCA2a relative to Sham hearts (B) <t>Microarray</t> based profiling of RNA isolated from rat hearts subjected to Myocardial infarction and rescue by Serca2a gene therapy. (C) qRT-PCR of Neat1 from RNA isolated from rat ventricle after MI and MI+Serca2a gene therapy. (D) qRT-PCR for detecting the change in expression of ANP, β-MHC, Neat1, and Neat1.2 in primary neonatal rat ventricular myocytes (NRCM) challenged with 100 µM Isoproterenol for 48 h. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between both groups. (E) Neat1 expression levels detected by qRT-PCR and ejection fraction (EF%) throughout heart failure progression (TAC, transverse aortic constriction; n = 5–8). * p value < 0.05, t-tests were performed to compute statistical differences between Sham and TAC. (F) Neat1 expression levels and ejection fraction (EF%) throughout heart failure progression after subjecting mice to 15 mg/kg/day, 30mg/kg/day, and 50 mg/kg/day of isoproterenol for 10 days. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between saline and isoproterenol groups. (G) qRT-PCR for detecting the expression of total Neat1 and Neat1.2 in adult mice cardiomyocytes and adult mice non-myocyte fraction.$
Rat Lncrna Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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riboarraytm rat lncrna array (Ribobio co)

Ribobio co riboarraytm rat lncrna array

Hierarchical clustering heat map revealing the distinct <t>lncRNA</t> expression profiles in the podocytes of the control and STZ-treated diabetic rats. After 6 weeks, the podocytes were obtained from control and STZ-treated diabetic rats and hierarchical clustering analysis was performed. The relative lncRNA expression is depicted according to the color scale. The red column indicates upregulation and the green column indicates downregulation. lncRNA, long noncoding RNA; STZ, streptozocin. Control 1, 2 and 3 indicated three representative podocytes samples from control rats; STZ 1, 2 and 3 indicated three representative podocytes samples from STZ-treated diabetic rats.

Riboarraytm Rat Lncrna Array, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+lncrna+array/riboarraytm+rat+lncrna+array/pmc05846646-88-15-20
Average 90 stars, based on 1 article reviews

riboarraytm rat lncrna array - by Bioz Stars, 2026-11

90/100 stars

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Image Search Results

$(A) Experimental setup to detect the deregulated lncRNA in hearts subjected to HF, HF+AAV9-SERCA2a relative to Sham hearts (B) Microarray based profiling of RNA isolated from rat hearts subjected to Myocardial infarction and rescue by Serca2a gene therapy. (C) qRT-PCR of Neat1 from RNA isolated from rat ventricle after MI and MI+Serca2a gene therapy. (D) qRT-PCR for detecting the change in expression of ANP, β-MHC, Neat1, and Neat1.2 in primary neonatal rat ventricular myocytes (NRCM) challenged with 100 µM Isoproterenol for 48 h. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between both groups. (E) Neat1 expression levels detected by qRT-PCR and ejection fraction (EF%) throughout heart failure progression (TAC, transverse aortic constriction; n = 5–8). * p value < 0.05, t-tests were performed to compute statistical differences between Sham and TAC. (F) Neat1 expression levels and ejection fraction (EF%) throughout heart failure progression after subjecting mice to 15 mg/kg/day, 30mg/kg/day, and 50 mg/kg/day of isoproterenol for 10 days. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between saline and isoproterenol groups. (G) qRT-PCR for detecting the expression of total Neat1 and Neat1.2 in adult mice cardiomyocytes and adult mice non-myocyte fraction.$

Journal: bioRxiv

Article Title: The long non-coding RNA NEAT1 regulates the transcriptional landscape of cardiomyocytes

doi: 10.1101/2024.06.27.600932

Figure Lengend Snippet: (A) Experimental setup to detect the deregulated lncRNA in hearts subjected to HF, HF+AAV9-SERCA2a relative to Sham hearts (B) Microarray based profiling of RNA isolated from rat hearts subjected to Myocardial infarction and rescue by Serca2a gene therapy. (C) qRT-PCR of Neat1 from RNA isolated from rat ventricle after MI and MI+Serca2a gene therapy. (D) qRT-PCR for detecting the change in expression of ANP, β-MHC, Neat1, and Neat1.2 in primary neonatal rat ventricular myocytes (NRCM) challenged with 100 µM Isoproterenol for 48 h. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between both groups. (E) Neat1 expression levels detected by qRT-PCR and ejection fraction (EF%) throughout heart failure progression (TAC, transverse aortic constriction; n = 5–8). * p value < 0.05, t-tests were performed to compute statistical differences between Sham and TAC. (F) Neat1 expression levels and ejection fraction (EF%) throughout heart failure progression after subjecting mice to 15 mg/kg/day, 30mg/kg/day, and 50 mg/kg/day of isoproterenol for 10 days. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between saline and isoproterenol groups. (G) qRT-PCR for detecting the expression of total Neat1 and Neat1.2 in adult mice cardiomyocytes and adult mice non-myocyte fraction.

Article Snippet: Rat lncRNA microarray (4X44K, Arraystar) was performed in the heart samples isolated from the rats that were subjected to proximal coronary artery ligation (HF), HF+ SERCA2A gene therapy.

Techniques: Microarray, Isolation, Quantitative RT-PCR, Expressing, Saline

Hierarchical clustering heat map revealing the distinct lncRNA expression profiles in the podocytes of the control and STZ-treated diabetic rats. After 6 weeks, the podocytes were obtained from control and STZ-treated diabetic rats and hierarchical clustering analysis was performed. The relative lncRNA expression is depicted according to the color scale. The red column indicates upregulation and the green column indicates downregulation. lncRNA, long noncoding RNA; STZ, streptozocin. Control 1, 2 and 3 indicated three representative podocytes samples from control rats; STZ 1, 2 and 3 indicated three representative podocytes samples from STZ-treated diabetic rats.

Journal: International Journal of Molecular Medicine

Article Title: Long noncoding RNA ENSRNOG00000037522 is involved in the podocyte epithelial-mesenchymal transition in diabetic rats

doi: 10.3892/ijmm.2018.3457

Figure Lengend Snippet: Hierarchical clustering heat map revealing the distinct lncRNA expression profiles in the podocytes of the control and STZ-treated diabetic rats. After 6 weeks, the podocytes were obtained from control and STZ-treated diabetic rats and hierarchical clustering analysis was performed. The relative lncRNA expression is depicted according to the color scale. The red column indicates upregulation and the green column indicates downregulation. lncRNA, long noncoding RNA; STZ, streptozocin. Control 1, 2 and 3 indicated three representative podocytes samples from control rats; STZ 1, 2 and 3 indicated three representative podocytes samples from STZ-treated diabetic rats.

Article Snippet: Next, cRNA was hybridized to the microarray overnight, and microarray profiling was performed using the RiboArrayTM Rat lncRNA Array ( http://www.ribobio.com/sitecn/Service2.aspx?id=104 ; RiboBio Co., Ltd. (Guangzhou, China) following the manufacturer's protocol.

Techniques: Expressing, Control

GO analysis of differentially expressed lncRNAs between the control and STZ-treated diabetic rats. Three domains were covered, including cellular component, molecular function and biological process. The GO analysis indicated that differentially expressed lncRNAs were mainly involved in the cell, cell part and cellular process. GO, Gene Ontology; lncRNA, long noncoding RNA; STZ, streptozocin.

Journal: International Journal of Molecular Medicine

Article Title: Long noncoding RNA ENSRNOG00000037522 is involved in the podocyte epithelial-mesenchymal transition in diabetic rats

doi: 10.3892/ijmm.2018.3457

Figure Lengend Snippet: GO analysis of differentially expressed lncRNAs between the control and STZ-treated diabetic rats. Three domains were covered, including cellular component, molecular function and biological process. The GO analysis indicated that differentially expressed lncRNAs were mainly involved in the cell, cell part and cellular process. GO, Gene Ontology; lncRNA, long noncoding RNA; STZ, streptozocin.

Techniques: Control

Kyoto Encyclopedia of Genes and Genomes pathway analysis of differentially expressed lncRNAs between the control and STZ-treated diabetic rats. The three most enriched pathways were 'pathways in cancer', 'PI3K-Akt signaling pathway' and 'microRNAs in cancer'. lncRNA, long noncoding RNA; STZ, streptozocin.

Journal: International Journal of Molecular Medicine

Article Title: Long noncoding RNA ENSRNOG00000037522 is involved in the podocyte epithelial-mesenchymal transition in diabetic rats

doi: 10.3892/ijmm.2018.3457

Figure Lengend Snippet: Kyoto Encyclopedia of Genes and Genomes pathway analysis of differentially expressed lncRNAs between the control and STZ-treated diabetic rats. The three most enriched pathways were 'pathways in cancer', 'PI3K-Akt signaling pathway' and 'microRNAs in cancer'. lncRNA, long noncoding RNA; STZ, streptozocin.

Techniques: Control

(A) RT-qPCR was used to validate the 7 selected lncRNAs transcripts. * P<0.05 vs. control group. (B) Subsequent to treatment of podocytes with high glucose, RT-qPCR validation was performed for the 7 selected lncRNA transcripts. lncRNA ENSRNOG00000037522 was the most upregulated lncRNA in podocytes from the high glucose treatment group compared with the normal group. * P<0.05 vs. normal cells. All experiments were performed in triplicate, and the results are presented as the mean ± standard deviation. lncRNA, long noncoding RNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Journal: International Journal of Molecular Medicine

Article Title: Long noncoding RNA ENSRNOG00000037522 is involved in the podocyte epithelial-mesenchymal transition in diabetic rats

doi: 10.3892/ijmm.2018.3457

Figure Lengend Snippet: (A) RT-qPCR was used to validate the 7 selected lncRNAs transcripts. * P<0.05 vs. control group. (B) Subsequent to treatment of podocytes with high glucose, RT-qPCR validation was performed for the 7 selected lncRNA transcripts. lncRNA ENSRNOG00000037522 was the most upregulated lncRNA in podocytes from the high glucose treatment group compared with the normal group. * P<0.05 vs. normal cells. All experiments were performed in triplicate, and the results are presented as the mean ± standard deviation. lncRNA, long noncoding RNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Techniques: Quantitative RT-PCR, Control, Biomarker Discovery, Standard Deviation, Reverse Transcription, Real-time Polymerase Chain Reaction

(A) The knockdown efficiency of the lncRNA ENSRNOG00000037522 was examined with reverse transcription-quantitative polymerase chain reaction, and a significant abrogation of lncRNA ENSRNOG00000037522 was observed following transfection with siRNA1 or siRNA2. (B) Western blot analysis was conducted to determine the α-SMA, vimentin, nephrin and POXLD1 expression levels in lncRNA ENSRNOG00000037522 knockdown and control podocytes. (C) Protein levels were quantified by ImageJ software and are presented in the histogram. Podocytes transfected with NC siRNA served as the controls. All experiments were performed in triplicate, and the results are presented as the mean ± standard deviation. * P<0.05 vs. NC group. lncRNA, long noncoding RNA; NC, negative control; siRNA, small interfering RNA.

Journal: International Journal of Molecular Medicine

Article Title: Long noncoding RNA ENSRNOG00000037522 is involved in the podocyte epithelial-mesenchymal transition in diabetic rats

doi: 10.3892/ijmm.2018.3457

Figure Lengend Snippet: (A) The knockdown efficiency of the lncRNA ENSRNOG00000037522 was examined with reverse transcription-quantitative polymerase chain reaction, and a significant abrogation of lncRNA ENSRNOG00000037522 was observed following transfection with siRNA1 or siRNA2. (B) Western blot analysis was conducted to determine the α-SMA, vimentin, nephrin and POXLD1 expression levels in lncRNA ENSRNOG00000037522 knockdown and control podocytes. (C) Protein levels were quantified by ImageJ software and are presented in the histogram. Podocytes transfected with NC siRNA served as the controls. All experiments were performed in triplicate, and the results are presented as the mean ± standard deviation. * P<0.05 vs. NC group. lncRNA, long noncoding RNA; NC, negative control; siRNA, small interfering RNA.

Techniques: Knockdown, Reverse Transcription, Real-time Polymerase Chain Reaction, Transfection, Western Blot, Expressing, Control, Software, Standard Deviation, Negative Control, Small Interfering RNA

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